Manipulating the Mouse Embryo

Manipulating the Mouse Embryo PDF Author: Richard Behringer
Publisher:
ISBN: 9781936113019
Category : MEDICAL
Languages : en
Pages : 0

Book Description
Enthält: Genetics and embryology of the mouse: past, present, and future. Summary of mouse development. A mouse colony for the production of transgenic and chimeric animals. Recovery and in vitro culture of preimplantation embryos. Isolation, culture, and manipulation of postimplantation embryos. Surgical procedures. Production of transgenic mice by pronuclear microinjection. Embryo-derived stem cell lines. Germ line-competent stem cells derived from adult mice. Vector designs for pluripotent stem cell-based transgenesis and genome alterations. Introduction of foreign DNA into embryonic stem cells. Production of chimeras. Genotyping. Parthenogenesis, pronuclear transfer, and mouse cloning. Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection. Cryopreservation, rederivation, and transport of mouse strains. Techniques for visualizing gene products, cells, tissues, and organ systems. Setting up a micromanipulation lab.

Manipulating the Mouse Embryo

Manipulating the Mouse Embryo PDF Author: Brigid Hogan
Publisher: Cold Spring Harbor Laboratory Press
ISBN:
Category : Nature
Languages : en
Pages : 352

Book Description
Of mouse development -- Setting up a colony for the production of transgenic mice -- Recovery, culture, and transfer of embryos -- Introduction of new genetic information into the developing mouse embryo -- Iolation of pluripotential stem cell lines -- Techhniques for visualizing genes and gene products -- In vitro culture of eggs, embryos, and teratocarcinoma cels -- Chemicals, supplies, and solutions.

小鼠胚胎操作实验指南

小鼠胚胎操作实验指南 PDF Author: A.·纳吉 (加)
Publisher:
ISBN: 9787030124388
Category : Mice
Languages : en
Pages : 764

Book Description
本书其余责任者还有:(加)M. 格斯滕斯坦、(德)K. 文特斯滕、(美)R. 贝林杰。

Manipulating the Mouse Embryo

Manipulating the Mouse Embryo PDF Author: Andras Nagy
Publisher: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press
ISBN:
Category : Science
Languages : en
Pages : 784

Book Description
Provides background information and detailed protocols for developing a mouse colony and using the animals in transgenic and gene-targeting experiments. The protocols list the animals, equipment, and reagents required and step-by-step procedures. Topics include in vitro culture of preimplantation embryos, surgical procedures, the production of chimeras, and the analysis of genome alterations. The third edition adds protocols for cloning mice, modifying embryonic stem cells, intracytoplasmic sperm injection, and cryopreservation of embryos.

Laboratory Manual

Laboratory Manual PDF Author: Commonwealth Scientific and Industrial Research Organization (Australia). Division of Animal Production
Publisher:
ISBN:
Category : Microinjections
Languages : en
Pages : 34

Book Description

Pseudokinases

Pseudokinases PDF Author:
Publisher: Academic Press
ISBN: 0323915426
Category : Science
Languages : en
Pages : 838

Book Description
Pseudokinases, Volume 667, the latest release in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting interesting chapters, including the Production and Purification of the PEAK pseudokinases for structural and functional studies, Structural biology and biophysical characterization of Tribbles pseudokinases, Detecting endogenous TRIB protein expression and its downstream signaling, Analysis of human Tribbles 2 pseudokinase, Expression, purification and examination of ligand-binding to IRAK pseudokinases, Characterization of pseudokinase ILK-mediated actin assembly, Biochemical examination of Titin pseudokinase, Approaches to study pseudokinase conformations, CRISPR editing cell lines for reconstitution studies of pseudokinase function, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in Methods in Enzymology serials Includes the latest information on Pseudokinases

A Laboratory Guide to the Mammalian Embryo

A Laboratory Guide to the Mammalian Embryo PDF Author: David K. Gardner
Publisher: Oxford University Press
ISBN: 9780195142266
Category : Medical
Languages : en
Pages : 434

Book Description
Never before has there been such a comprehensive book of protocols. This compendium offers a full range of research techniques-from cell culture, to biochemical, to microscopic and genetic. More focused books, like Cold Spring Harbor's Manipulating the Mouse Embryo, are similar though more narrow in scope. This book will appeal to a broad range of researchers, from basic experimental scientists to clinical and animal scientists.

Laboratory Animal Medicine

Laboratory Animal Medicine PDF Author: Lynn C. Anderson
Publisher: Elsevier
ISBN: 012416613X
Category : Technology & Engineering
Languages : en
Pages : 1727

Book Description
Laboratory Animal Medicine, Third Edition, is a fully revised publication from the American College of Laboratory Medicine’s acclaimed blue book series. It presents an up-to-date volume that offers the most thorough coverage of the biology, health, and care of laboratory animals. The book is organized by species, with new inclusions of chinchillas, birds, and program and employee management, and is written and edited by known experts in the fields. Users will find gold-standard guidance on the study of laboratory animal science, as well as valuable information that applies across all of the biological and biomedical sciences that work with animals. Organized by species for in-depth understanding of biology, health, and best care of animals Features the inclusion of chinchillas, quail, and zebra finches as animal models Offers guidance on program and employee management Covers regulations, policies, and laws for laboratory animal management worldwide
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